How to set the Read file Access Denied using C# dot net

How to set the Read file Access Denied using C# dot net

string filename = @"c:\karthi\text\bb.txt";
System.Security.Permissions.FileIOPermission permission = new
System.Security.Permissions.FileIOPermission(System.Security.Permissions.FileIOPermissionAccess.Read ,filename);

System.IO.StreamReader sr = new System.IO.StreamReader(filename);

catch (System.Security.SecurityException ex)
MessageBox.Show("Permission to read the file was denied " );
catch (System.Exception ex)

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