DataSource of the GridView Like : 

        DataTable dt = new DataTable();
        dt.Columns.Add("FilePath");

        DataRow dr = dt.NewRow();
        dr[0] = "~//Images/DSC00573.JPG";
        dt.Rows.Add(dr);
        dr = dt.NewRow();
        dr[0] = "~//Images/DSC00574.JPG";
        dt.Rows.Add(dr);

        GridView1.DataSource = dt;
        GridView1.DataBind();