<script type="text/javascript"> 
function over(t,id) 
    {
    //col
    var x 
    var mycol = getElementsById(t,id) 
    for (x in mycol) 
    {
            if (mycol[x].style.backgroundColor != "red")
            mycol[x].style.backgroundColor = "#ccffcc"; 
    }
	}

function out(t,id) 
{
    //col
    var x 
    var mycol = getElementsById(t,id) 
    for (x in mycol) 
    {
        if (mycol[x].style.backgroundColor != "red")
        mycol[x].style.backgroundColor = "white"; 
    }
 }
 

function getElementsById(sId,id) 
{

var outArray = new Array(); 

//rows
var dt = document.getElementById("dtgView");
for(var j = 0; j < dt.rows.length; j++)
{
    outArray[j] = document.all[sId][j];
}

//columns
var d;
var cId;

for (var k=0;k<document.all[sId].length-2;k+=1)
{
cId="TestCol" + k;
d = k + (dt.rows.length - 1);
outArray[d] = document.all[cId][id];
}

return outArray; 
}
</script>
<asp:datagrid id="dtgView" AutoGenerateColumns="true" Runat="server"></asp:datagrid>

Private Sub Page_Load(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles MyBase.Load
        'Put user code to initialize the page here        Dim d As DataTable
        dtgView.DataSource = d 'Datagrid bind code goes here. d is datasource. do code for this.
        dtgView.DataBind()
        
    End Sub

Private Sub dtgView_ItemDataBound(ByVal sender As Object, ByVal e As System.Web.UI.WebControls.DataGridItemEventArgs) Handles dtgView.ItemDataBound
        If e.Item.ItemType <> ListItemType.Header Then


            Dim k As Integer = 0
            For k = 0 To e.Item.Cells.Count - 1
                Dim id As String = "TestCol" & k
                e.Item.Cells(k).Attributes.Add("id", id)
                e.Item.Cells(k).Attributes.Add("onmouseover", "over('" & id & "'," & e.Item.ItemIndex & ")")
                e.Item.Cells(k).Attributes.Add("onmouseout", "out('" & id & "'," & e.Item.ItemIndex & ")")
            Next

        End If

    End Sub